Description
Carl Zeiss FLUAR 20x/0.75 ∞/0.17 Microscope Objective (420150-9900, M27)
Used, in perfect optical condition
The Zeiss FLUAR 20x/0.75 is a high-performance, high-numerical-aperture dry objective engineered for demanding fluorescence, confocal imaging, and laser microdissection. Built with specialized low-autofluorescence optical glass, it delivers exceptional transmission from the near-UV through the near-IR spectrum (400–800 nm throughput peak at 85%–98%). Designed for the modern Zeiss M27 thread mount and Infinity Color-Corrected System (ICS), it offers maximum photon capture without requiring immersion media.
Key Highlights
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Exceptional UV/VIS/NIR Transmission: 5-star rating for Ultra-Violet transmission; steep transmission curve starting below 340 nm and maintaining over 90% across the visible spectrum.
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High-NA Dry Convenience: Combines a high 0.75 numerical aperture with a 0.60 mm working distance, providing sharp, high-resolution imaging without oil or water immersion.
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Confocal & Laser Microdissection Ready: Optimized for UV/VIS laser confocal microscopy and laser microdissection workflows.
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Large Field of View: Supports up to 23 mm field of view for high-throughput screening and widefield observation.
Technical Specifications
| Specification |
Details |
| Model / Part Number |
Zeiss FLUAR 20x / 420150-9900 |
| Magnification |
20x |
| Numerical Aperture (NA) |
0.75 |
| Free Working Distance (WD) |
0.60 mm |
| Coverglass Correction |
0.17 mm |
| Immersion Medium |
Dry (Without Immersion) |
| Field of View (FOV) |
23 mm |
| Parfocal Length |
45.06 mm |
| Thread Standard |
M27 × 0.75 |
| Optical System |
ICS (Infinity Color Corrected System) |
Recommended Applications
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UV-excited fluorophores (DAPI, Hoechst, Alexa Fluor 350, Fura-2 ratiometric imaging)
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Laser Scanning Confocal Microscopy (CLSM)
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Laser-Assisted Microdissection (LMD / PALM)
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High-contrast Brightfield and Differential Interference Contrast (DIC)
